Group of Oligonucleotides
DEPARTMENT OF BIOLOGICAL CHEMISTRY
Institute of Organic Chemistry and Biochemistry, Prague, Czech Republic
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The main goal of the Group is the investigation of nuclease-resistant oligonucleotides in deoxyribo and ribo series containing structurally diverse P-C bridging internucleotide linkages, with the aim to bring a new quality into the family of antisense and triplex forming (TFO) oligonucleotides, aptamers and other compounds interfering with gene expression. The research involves (i) the design and chemical synthesis of novel types isopolar isosteric and nonisosteric phosphonate analogues of nucleotides, (ii) the solid-phase synthesis of chimeric oligonucleotides, (iii) the study on physico-chemical and biological properties of modified oligonucleotides, e.g. conformation of altered sugar-phosphate backbone, hybridization properties, nucleases resistance, ability to elicit RNase H activity, cellular uptake, etc. Contact: Ivan Rosenberg Institute of Organic Chemistry and Biochemistry Academy of Sciences of the Czech Republic Flemingovo n. 2 CZ-166 10 Prague 6 Czech Republic, Europe Phone: +420 220183381 Fax: +420 233335268 E-mail: ivan@uochb.cas.cz |