Novel types of modified derivatives and analogues of nucleobases, nucleosides, nucleotides and nucleic acids are designed and prepared for applications in all areas of biomedicinal sciences (medicinal chemistry, biochemistry, chemical biology, bioanalysis etc.).
Basic developments of methodology for the synthesis of these modified biomolecules are performed largely using modern methods (including metal- or enzyme-catalyzed reactions). Biological activity (cytostatic, antiviral etc.) of the novel nucleobases, nucleosides and nucleotides is systematically studied in collaboration with several groups both in academia and in pharmaceutical industry. Several collaborative projects also address chemical biology of nucleic acids (i.e. DNA polymerase specificity and fidelity). Functionalized nucleic acids bearing diverse interesting and useful substituents are another goal of our group. For details, see Projects.
Kielkowski, P. et al: "Nucleobase Protection Strategy for Gene Cloning and Expression" ChemBioChem 2013, 14, 801-804.
Ménová, P. et al: "Polymerase synthesis of oligonucleotides containing a single chemically modified nucleobase for site-specific redox labelling" Chem. Commun. 2013, 49, 4652-4654.
Riedl, J. et al: "Labelling of nucleosides and oligonucleotides by solvatochromic 4-aminophthalimide fluorophore for studying DNA–protein interactions" Chem. Sci. 2012, 3, 2797–2806.